turbidometric platelet aggregometer Search Results


turbidometric platelet aggregometer  (Chrono-log corporation)
90
Chrono-log corporation turbidometric platelet aggregometer
The role of Akt in GPIb-IX–mediated platelet aggregation and the PI3K-dependent Akt activation induced by vWF. (A) Washed wild-type (WT) and Akt1−/− mouse platelets were stimulated with botrocetin (2 μg/mL) and vWF (7.5 μg/mL). Platelet aggregation was monitored using a <t>turbidometric</t> <t>aggregometer</t> at 37°C and 1000 rpm stirring speed. (B) WT and Akt1−/− platelets were treated with DMSO or SH-6 (15 μM) for 2 minutes and then stimulated with botrocetin (1.2 μg/mL) and vWF (10 μg/mL). (C) WT and Akt2−/− platelets were stimulated with botrocetin and vWF as in panel B. (D) Washed human platelets were preincubated with Akt inhibitor SH-6 (15 μM) or the vehicle (DMSO) for 2 minutes, and then stimulated with botrocetin and vWF as in panel B. (E) Washed human platelets (5 × 108/mL) were preincubated with DMSO, PI3K inhibitors, wortmannin (100 nM), or LY294002 (20 μM), then stimulated with ristocetin (0.5 mg/mL) in the absence or presence of 25 μg/mL vWF for 1 minute in a platelet aggregometer. Platelets were solubilized and immunoblotted with a rabbit antibody specifically recognizing Akt phosphorylated at serine-473 (p-Akt) or with a rabbit anti-Akt antibody to indicate loading levels (Akt).
Turbidometric Platelet Aggregometer, supplied by Chrono-log corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/turbidometric+platelet+aggregometer/turbidometric+platelet+aggregometer/pmc02200862-47-23-26
Average 90 stars, based on 1 article reviews
turbidometric platelet aggregometer - by Bioz Stars, 2026-09
90/100 stars
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turbidometric platelet aggregometer 560 ca  (Chrono-log corporation)
90
Chrono-log corporation turbidometric platelet aggregometer 560 ca
The role of Akt in GPIb-IX–mediated platelet aggregation and the PI3K-dependent Akt activation induced by vWF. (A) Washed wild-type (WT) and Akt1−/− mouse platelets were stimulated with botrocetin (2 μg/mL) and vWF (7.5 μg/mL). Platelet aggregation was monitored using a <t>turbidometric</t> <t>aggregometer</t> at 37°C and 1000 rpm stirring speed. (B) WT and Akt1−/− platelets were treated with DMSO or SH-6 (15 μM) for 2 minutes and then stimulated with botrocetin (1.2 μg/mL) and vWF (10 μg/mL). (C) WT and Akt2−/− platelets were stimulated with botrocetin and vWF as in panel B. (D) Washed human platelets were preincubated with Akt inhibitor SH-6 (15 μM) or the vehicle (DMSO) for 2 minutes, and then stimulated with botrocetin and vWF as in panel B. (E) Washed human platelets (5 × 108/mL) were preincubated with DMSO, PI3K inhibitors, wortmannin (100 nM), or LY294002 (20 μM), then stimulated with ristocetin (0.5 mg/mL) in the absence or presence of 25 μg/mL vWF for 1 minute in a platelet aggregometer. Platelets were solubilized and immunoblotted with a rabbit antibody specifically recognizing Akt phosphorylated at serine-473 (p-Akt) or with a rabbit anti-Akt antibody to indicate loading levels (Akt).
Turbidometric Platelet Aggregometer 560 Ca, supplied by Chrono-log corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/turbidometric+platelet+aggregometer/turbidometric+platelet+aggregometer+560+ca/us07932233-40-5-8
Average 90 stars, based on 1 article reviews
turbidometric platelet aggregometer 560 ca - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
turbidometric platelet aggregometer  (Bio/Data Inc)
90
Bio/Data Inc turbidometric platelet aggregometer
The role of Akt in GPIb-IX–mediated platelet aggregation and the PI3K-dependent Akt activation induced by vWF. (A) Washed wild-type (WT) and Akt1−/− mouse platelets were stimulated with botrocetin (2 μg/mL) and vWF (7.5 μg/mL). Platelet aggregation was monitored using a <t>turbidometric</t> <t>aggregometer</t> at 37°C and 1000 rpm stirring speed. (B) WT and Akt1−/− platelets were treated with DMSO or SH-6 (15 μM) for 2 minutes and then stimulated with botrocetin (1.2 μg/mL) and vWF (10 μg/mL). (C) WT and Akt2−/− platelets were stimulated with botrocetin and vWF as in panel B. (D) Washed human platelets were preincubated with Akt inhibitor SH-6 (15 μM) or the vehicle (DMSO) for 2 minutes, and then stimulated with botrocetin and vWF as in panel B. (E) Washed human platelets (5 × 108/mL) were preincubated with DMSO, PI3K inhibitors, wortmannin (100 nM), or LY294002 (20 μM), then stimulated with ristocetin (0.5 mg/mL) in the absence or presence of 25 μg/mL vWF for 1 minute in a platelet aggregometer. Platelets were solubilized and immunoblotted with a rabbit antibody specifically recognizing Akt phosphorylated at serine-473 (p-Akt) or with a rabbit anti-Akt antibody to indicate loading levels (Akt).
Turbidometric Platelet Aggregometer, supplied by Bio/Data Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/turbidometric+platelet+aggregometer/turbidometric+platelet+aggregometer/us08101077-264-34-33
Average 90 stars, based on 1 article reviews
turbidometric platelet aggregometer - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


The role of Akt in GPIb-IX–mediated platelet aggregation and the PI3K-dependent Akt activation induced by vWF. (A) Washed wild-type (WT) and Akt1−/− mouse platelets were stimulated with botrocetin (2 μg/mL) and vWF (7.5 μg/mL). Platelet aggregation was monitored using a turbidometric aggregometer at 37°C and 1000 rpm stirring speed. (B) WT and Akt1−/− platelets were treated with DMSO or SH-6 (15 μM) for 2 minutes and then stimulated with botrocetin (1.2 μg/mL) and vWF (10 μg/mL). (C) WT and Akt2−/− platelets were stimulated with botrocetin and vWF as in panel B. (D) Washed human platelets were preincubated with Akt inhibitor SH-6 (15 μM) or the vehicle (DMSO) for 2 minutes, and then stimulated with botrocetin and vWF as in panel B. (E) Washed human platelets (5 × 108/mL) were preincubated with DMSO, PI3K inhibitors, wortmannin (100 nM), or LY294002 (20 μM), then stimulated with ristocetin (0.5 mg/mL) in the absence or presence of 25 μg/mL vWF for 1 minute in a platelet aggregometer. Platelets were solubilized and immunoblotted with a rabbit antibody specifically recognizing Akt phosphorylated at serine-473 (p-Akt) or with a rabbit anti-Akt antibody to indicate loading levels (Akt).

Journal:

Article Title: The role of Akt in the signaling pathway of the glycoprotein Ib-IX-induced platelet activation

doi: 10.1182/blood-2007-04-085514

Figure Lengend Snippet: The role of Akt in GPIb-IX–mediated platelet aggregation and the PI3K-dependent Akt activation induced by vWF. (A) Washed wild-type (WT) and Akt1−/− mouse platelets were stimulated with botrocetin (2 μg/mL) and vWF (7.5 μg/mL). Platelet aggregation was monitored using a turbidometric aggregometer at 37°C and 1000 rpm stirring speed. (B) WT and Akt1−/− platelets were treated with DMSO or SH-6 (15 μM) for 2 minutes and then stimulated with botrocetin (1.2 μg/mL) and vWF (10 μg/mL). (C) WT and Akt2−/− platelets were stimulated with botrocetin and vWF as in panel B. (D) Washed human platelets were preincubated with Akt inhibitor SH-6 (15 μM) or the vehicle (DMSO) for 2 minutes, and then stimulated with botrocetin and vWF as in panel B. (E) Washed human platelets (5 × 108/mL) were preincubated with DMSO, PI3K inhibitors, wortmannin (100 nM), or LY294002 (20 μM), then stimulated with ristocetin (0.5 mg/mL) in the absence or presence of 25 μg/mL vWF for 1 minute in a platelet aggregometer. Platelets were solubilized and immunoblotted with a rabbit antibody specifically recognizing Akt phosphorylated at serine-473 (p-Akt) or with a rabbit anti-Akt antibody to indicate loading levels (Akt).

Article Snippet: 33 In some experiments, platelets were washed and resuspended according to Liu et al. 35 Platelet aggregation Platelet aggregation was measured in a turbidometric platelet aggregometer (Chronolog, Havertown, PA) at 37°C with stirring (1000 rpm).

Techniques: Activation Assay

The role of Akt in GPIb-IX–dependent elevation of platelet cGMP. Washed WT, Akt1−/−, and Akt2−/− platelets were stimulated with botrocetin alone or botrocetin (1.5 μg/mL) and vWF (10 μg/mL) in the aggregometer for 8 minutes. The cGMP levels (mean ± SD) in platelets were measured as described in “vWF-induced platelet cGMP elevation.” Data were obtained from 3 tests.

Journal:

Article Title: The role of Akt in the signaling pathway of the glycoprotein Ib-IX-induced platelet activation

doi: 10.1182/blood-2007-04-085514

Figure Lengend Snippet: The role of Akt in GPIb-IX–dependent elevation of platelet cGMP. Washed WT, Akt1−/−, and Akt2−/− platelets were stimulated with botrocetin alone or botrocetin (1.5 μg/mL) and vWF (10 μg/mL) in the aggregometer for 8 minutes. The cGMP levels (mean ± SD) in platelets were measured as described in “vWF-induced platelet cGMP elevation.” Data were obtained from 3 tests.

Article Snippet: 33 In some experiments, platelets were washed and resuspended according to Liu et al. 35 Platelet aggregation Platelet aggregation was measured in a turbidometric platelet aggregometer (Chronolog, Havertown, PA) at 37°C with stirring (1000 rpm).

Techniques: